(Mentor: Daniel Mendoza, Atmospheric Sciences)Įlectric Bus Air Quality Monitoring Platform: Findings and Implications Gabriel Baffour, University of Utah & Jessica Cuello, University of Utah The study is in its elementary stages, therefore results have not been finalized.
Methods include collecting air quality data within participant homes and implementing home-improvements, including but not limited to installing H-VAC systems and reinforcing shelter features. The study’s objective is to assess whether in-home interventions reduce air pollution and improve participant health. Poor air quality has been linked to adverse health outcomes such as higher rates of cancer, heart disease, stroke and respiratory diseases. Our air pollution consists of particulate matter (PM2.5), ozone and arsenic, which come from vehicle, home and industrial emissions. The Salt Lake Valley is notorious for its air pollution, which occurs when harmful matter enters the atmosphere. Interventional Assessment Of Air Quality On Low-Income Homes In Salt Lake County The FFPE protocol will be utilized in follow-up studies to characterize and isolate low-level pathogen signatures from environmental specimens.Ĭamden Alexander, University of Utah & Monica Owens, University of Utah FFPE specimen processing rendered higher levels of RNA and similar RNA integrity scores as compared to the O.C.T. Both the FFPE and OCT provided an efficient RNA integrity score required for downstream NGS processing. For FFPE, the 48hr fixed tissue had a higher RNA concentration than the 24hr fixed tissue. For the OCT protocol, the RNA concentrations were lower than for the FFPE protocol. protocols was used to determine the optimal RNA concentrations and integrity for 24hr and 48hr fixed tissues. The RNA isolation using the FFPE and O.C.T. protocol required less time and reagents to process samples. FFPE required longer sample processing time, more reagents for step-wise tissue dehydration, and instrumentation to facilitate embedding specimens into hot paraffin wax. Each protocol had its own timeframe, challenges, and implications. This study focused on the FFPE and O.C.T. The objective of this study was to determine the optimal protocol for isolation of RNA from environmental samples. RNA quantity was measured with Qubit™ RNA High Sensitivity (HS) Assay Kit and RNA integrity was assessed using Qubit™ RNA IQ Assay Kit. RNA was then isolated from 5um thick slices using a Qiagen RNeasy FFPE Kit. Tissues were fixed with 10% formalin for 24hr or 48hr prior to being embedded using either the FFPE or the O.C.T. A study was designed evaluated two protocols for specimen preparation for optimized preservation and integrity of cellular and viral RNA: 1) formalin-fixed paraffin embedded (FFPE) sample processing and 2) optimal cutting temperature (O.C.T.) embedding compound for fixed and frozen tissue specimens. Optimizing Scientific Protocols for RNA Isolation From Fixed Tissueĭetection of pathogens in environmental specimens is challenging due to the rapid degradation of nucleic acids based on preservation techniques used.
RISK PREVALENCE FOR TYPE 2 DIABETES PILOT STUDY PDF SERIES
Undergraduate Research Education Series (URES).Undergraduate Research Scholar Designation.Summer Program for Undergraduate Research (SPUR).Undergraduate Research Opportunity Program (UROP).
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